Nondenaturing procedure for rapid preparation of ferredoxin from Clostridium pasteurianum.

Lovcnberg and Williams (1‘1 have reported that, when a solution of Clostridium pastewianum ferredoxin in 90% ammonium sulfate is treated on a column of DEAE-cellulose, ferredoxin is retained by the column. They showed t,hat, ferredoxin is eluted when the ammoniuni sulfate concentration is lowered to about 35c/, saturation. Thcsc observations suggested the method described in this paper for purifying ferredoxin from crude extracts of (7. pnstewimum. This novel procedure provides pure ferredoxin in yields comparable with those obtained using a method described earlier by Mortenson (2’). Since it, avoids the strongly denaturing conditions that occur during the initial acetone fractionation of Mortenson’s method (2), this proccclure may bc csperially useful when other proteins arc to be prc’parccl from the same starting material.